Introduction
In this experiment, we will be doing the analysis of the various type of solvents that can be used to extract the bioactive compounds from Arctostaphylos.
Aim
To find the best solvent for extracting bioactive compounds in Arctostaphylos.
Theory
1. The plant genus containing bearberries and manzanita is termed Arctostaphyuls.
2. Arctostaphylos are shrubs of small trees that have around 60 species all around the globe.
Requirements
1. Mortar
2. Pestle
3. Arctostaphylos plant
4. Agar plate inoculated with E.coli.
5. Fresh garlic
7. DMSO
8. Water
9. Ethanol
10. Acetone
11. Notebook
12. Pen or Pencil
13. A concentration disk
Procedure
Step 1: To test the extraction of the antibiotic compounds, use five different solvents such Water, DMSO, Ethanol, Acetone, and Isopropyl Alcohol.
Step 2: Using a pestle and a mortar, grind the solvent with the Arctostaphylos plant. This will give you five different samples.
Step 3: Take a concentration disk and soak it in one of the samples.
Step 4: Place the soaked disk on an agar plate.
Step 5: Take fresh garlic and use it as a positive inhibitory control.
Step 6: Use pure solvent as a negative control.
Step 7: Incubate the agar plate at 84 degrees Celsius.
Step 8: Repeat the experiment with each of the samples.
Step 9: Measure the degree of extraction by measuring the distance between the control disk’s ring and the inhibition’s ring for the best concentration disk.
Step 10: Perform a retesting for the three solvents that has the largest ring difference.
Step 11: Record your observations.
Observation
1. In our test of analysis of the bioactive compounds in Arctostaphylos in Various Solvents, we observed that Isopropyl alcohol had the largest ring difference, followed by DMSO, Water, Ethanol, and Acetone.
2. While in the retesting, we saw that DMSO acquired the largest ring difference. After this came Water and Isopropyl alcohol.
Result
1. The bioactive compound Manzanita dissolved more in DMSO than in water.
2. Acetone and Ethanol did not dissolve the compound well.
3. On the other hand, Isopropyl Alcohol showed opposite results in both the tests.
4. In the first test, it dissolved Manzanita and was the best solvent. While in the second test, it was the worst.
Precautions
1. Handle the plant with care.
2. Use chemicals in small amounts.
Conclusion
In this experiment, we have determined the best solvent that can be used for extracting bioactive compounds from Arctostaphylus to avoid the entry of antimicrobial agents in the plant.
Viva Questions With Answers
Q.1 What was the aim of your experiment?
ANS. We aimed to determine the best solvent for extracting bioactive compounds in Arctostaphylos.
Q.2 Which solvent was the best for extracting bioactive compounds from Arctostaphylos?
ANS. The solvent DMSO was the best for extracting bioactive compounds from Arctostaphylos.
Q.3 What is the full form of DMSO?
ANS. Dimethyl Sulfoxide
Saquib Siddiqui is a Mechanical Engineer and a content creator. He has special interest in science experiments and teaches the same to school and college students. He is also a technical lead at RUDE LABS PVT. LTD.
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