Introduction
In this experiment, we will see that carbon dioxide is released during the process of respiration.
Basic Building Concept
1. Respiration is the process of inhaling oxygen and exhaling carbon dioxide.
2. It is of two types:
a. Aerobic Respiration.
b. Anaerobic Respiration.
Aerobic Respiration
1. The breaking down of food in the presence of oxygen is called aerobic respiration.
2. In this type of respiration maximum amount of energy is released by the complete breakdown of glucose.
3. The three steps which are involved in this type of respiration include-
Glycolysis, Kreb Cycle, and ETS.
4. The processes associated with glycolysis occur in the cytoplasm, and all the processes related to the Kreb cycle and ETS take place in mitochondria.
5. Equation for representing aerobic respiration is:
C6H12O6 + 6O2 → 6CO2 + 6H2O + Energy(ATP)
Anaerobic Respiration
1. The breaking down of food in the absence of oxygen is called aerobic respiration.
2. In this type of respiration, the minimum amount of energy is released by the incomplete breakdown of glucose.
3. Equation for representing anaerobic respiration is:
Yeast
C6H12O6 → 2C2H5OH + 2CO2 + Energy (ATP)
Glucose Ethyl Alcohol
Muscle cell
C6H12O6 → 2C3H6O6 + Energy (ATP)
Lactic Acid
Aim
To show that carbon dioxide is given out during respiration.
Requirements
1. U-shaped delivery tube,
2. Conical flask,
3. Blotting paper,
4. Thread,
5. Beaker,
6. Test tube,
7. Water,
8. Rubber cork with one hole,
9. 20% freshly prepared potassium hydroxide solution,
10. Soaked gram seeds,
11. Vaseline.
Theory
1. Respiration is the breaking down of food or glucose to release energy.
2. We have taken gram seeds to perform this experiment because it releases carbon dioxide, which is absorbed by the potassium hydroxide solution potassium carbonate solution.
2KOH + CO2 → K2CO3 + H2O
Potassium hydroxide Potassium carbonate
Procedure
Step 1: Place twenty-five seeds of a gram in a moist blotting paper for three to four days to germinate them.
Step 2: Now, take out the seeds and place them in a conical flask, then sprinkle some water over them to moisten them.
Step 3: With the help of a thread, hang the 20% solution of potassium hydroxide in the conical flask.
Step 4: Place the rubber cork over the mouth of the conical flask.
Step 5: Place one end of the U-shaped delivery tube into the hole of a conical flask and the other end into a beaker containing water.
Step 6: To make the experiment air-tight, seal all the connections of the experiment through vaseline.
Step 7: Leave the setup undisturbed for 1-2 hours. After this, check the change in the level of water in the delivery tube.
Observation
The end of the delivery tube, which was dipped into the water, showed an increment in the level of water.
Result
The change in the level of water in the delivery tube is due to the vacuum created in the conical flask. When gram seeds release carbon dioxide, it gets absorbed by the solution of potassium hydroxide. This creates a vacuum in the conical flask and causes the water to move upward in the delivery tube. Hence its shows that carbon dioxide is released during the process of respiration.
Precautions
1. Use freshly prepared potassium hydroxide solution.
2. Don’t let the germinated seeds dry.
3. Make sure that all the connections have been sealed properly.
4. Hang the solution of KOH carefully into the conical flask.
Conclusion
Through this experiment, we have seen that carbon dioxide is released during respiration.
VIVA Questions With Answers
Q.1 What was the aim of our experiment?
ANS. To show that carbon dioxide is released during respiration.
Q.2 What do you understand about respiration?
ANS. Respiration is the process of inhaling oxygen and exhaling carbon dioxide.
Q.3 What do you know about aerobic respiration?
ANS. The breaking down of food in the presence of oxygen is known as aerobic respiration.
Q.4 Give the equation which shows aerobic respiration
ANS. C6H12O6 + 6O2 → 6CO2 + 6H2O + Energy(ATP)
Q.5 What do you understand about anaerobic respiration?
ANS. The breaking down of food in the absence of oxygen is known as anaerobic respiration.
Q.6 Give an equation that shows anaerobic respiration?
ANS. Yeast
C6H12O6 → 2C2H5OH + 2CO2 + Energy (ATP)
Glucose Ethyl Alcohol
Muscle cell
C6H12O6 → 2C3H6O6 + Energy (ATP)
Lactic Acid
Q.7 Which kind of respiration gives out maximum energy?
ANS. Aerobic respiration.
Q.8 What are the steps involved in aerobic respiration?
ANS. Glycolysis, Kreb Cycle, and ETS.
Q.9 Events related to glycolysis take place in which part of the cell?
ANS. Cytoplasm
Q.10 Events related to the Kreb cycle and ETS take place in which part of the cell?
ANS. Mitochondria
Saquib Siddiqui is a Mechanical Engineer with expertise in science projects and experiments. Saquib’s work focuses on integrating scientific concepts with practical applications, making complex ideas accessible and exciting for learners of all ages. In addition to his practical work, Saquib has authored several articles, research papers, and educational materials.
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